Percoll PLUS溶液
貨號：GCX175445
規格：250ml
Product Description
Percoll is a classic medium for density gradient
centrifugation of cells, viruses, and subcellular particles .
Percoll consists of colloidal silica particles of 15–30 nm diameter (23% w/w in water), which have been coated
with polyvinylpyrrolidone (PVP).The PVP coating renders the product completely non-toxic and ideal for
use with biological materials.The PVP is firmly bound to the silica particles as a monomolecular layer. Due to
its heterogeneity in particle size, sedimentation occurs at different rates, spontaneously creating very smooth,
isometric gradients in the range of 1.0– 1.3 g/ml. Most biological particles having sedimentation coefficient
values greater than 60S can be successfully isolated in Percoll gradients.
Centrifugation allowing mixing of large sample Percoll PLUS is also a silica-based colloidal medium for
cell separation by density gradient centrifugation. The silica particles of this medium are covalently coated
with silane, providing product stability and long shelf life. The silane coating also provides low osmolality and
toxicity, as well as low viscosity. Percoll PLUS has low levels of endotoxins, making it well-suited for cell
separation in clinical research applications. Cell separation is performed using the same conditions as
the original Percoll. The low toxicity of Percoll and Percoll PLUS ensures that removal of the medium from
separated cellular particles is not usually necessary.
